tie light microscope Search Results


tie inverted light microscope  (Nikon)
90
Nikon tie inverted light microscope
Tie Inverted Light Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tie light microscope  (Nikon)
90
Nikon tie light microscope
Tie Light Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tie light microscope - by Bioz Stars, 2026-03
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high frequency patterned excitation light  (Nikon)
99
Nikon high frequency patterned excitation light
High Frequency Patterned Excitation Light, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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light microscopy nikon eclipse tie  (Nikon)
90
Nikon light microscopy nikon eclipse tie
Light Microscopy Nikon Eclipse Tie, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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spectrax light source  (Lumencor Inc)
90
Lumencor Inc spectrax light source
Spectrax Light Source, supplied by Lumencor Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
spectrax light source - by Bioz Stars, 2026-03
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differential interference contrast (dic) microscopy  (Nikon)
90
Nikon differential interference contrast (dic) microscopy
The CSPG4 and perlecan interaction is involved in cell adhesion and spreading. (A) MM200 cell adhesion on fibronectin coated surface was used as a positive control (i). MM200 cell adhesion on HCAEC perlecan coated surfaces, in the absence of antibody (ii), in the presence of antibodies against α2β1 integrin clone BHA2.1 (iii), against perlecan clone A74 (iv) and against CSPG4 clones B5 (v) and clone 9.2.27 (vi). Cells were stained for actin filaments with Rhodamine-phalloidin (i–vi) and imaged using confocal <t>microscopy.</t> White arrows point out the polymerized actin filaments. Scale bar represents 10 µm. (B) Live cell images using <t>DIC</t> were also taken after MM200 cell adhesion on HCAEC perlecan coated surfaces in the absence of antibody (i), in the presence of antibody against CSPG4 clones B5 (ii) and clone 9.2.27 (iii) for 1 h. Scale bar represents 50 µm. (C) Analysis of the perimeter of cells exposed to the perlecan coating for 1 h in the absence or presence of antibodies from (B). A minimum of 10 cells were analysed from three confocal images taken at random for each condition. Individual data points are indicated as well as mean ± standard deviation. (D) The number of cells adhered to the perlecan coating after 1 h in the absence or presence of anti-CSPG4 antibodies determined from the live cell DIC images in (C). Individual data points were indicated as well as mean ± standard deviation. ‘*’ denoted significant differences compared to cells plated on perlecan in the absence of antibodies (P < 0.05) analysed by one-way ANOVA. Data shown are representative of three independent experiments.
Differential Interference Contrast (Dic) Microscopy, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
differential interference contrast (dic) microscopy - by Bioz Stars, 2026-03
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light microscope nikon tie  (Nikon)
90
Nikon light microscope nikon tie
The CSPG4 and perlecan interaction is involved in cell adhesion and spreading. (A) MM200 cell adhesion on fibronectin coated surface was used as a positive control (i). MM200 cell adhesion on HCAEC perlecan coated surfaces, in the absence of antibody (ii), in the presence of antibodies against α2β1 integrin clone BHA2.1 (iii), against perlecan clone A74 (iv) and against CSPG4 clones B5 (v) and clone 9.2.27 (vi). Cells were stained for actin filaments with Rhodamine-phalloidin (i–vi) and imaged using confocal <t>microscopy.</t> White arrows point out the polymerized actin filaments. Scale bar represents 10 µm. (B) Live cell images using <t>DIC</t> were also taken after MM200 cell adhesion on HCAEC perlecan coated surfaces in the absence of antibody (i), in the presence of antibody against CSPG4 clones B5 (ii) and clone 9.2.27 (iii) for 1 h. Scale bar represents 50 µm. (C) Analysis of the perimeter of cells exposed to the perlecan coating for 1 h in the absence or presence of antibodies from (B). A minimum of 10 cells were analysed from three confocal images taken at random for each condition. Individual data points are indicated as well as mean ± standard deviation. (D) The number of cells adhered to the perlecan coating after 1 h in the absence or presence of anti-CSPG4 antibodies determined from the live cell DIC images in (C). Individual data points were indicated as well as mean ± standard deviation. ‘*’ denoted significant differences compared to cells plated on perlecan in the absence of antibodies (P < 0.05) analysed by one-way ANOVA. Data shown are representative of three independent experiments.
Light Microscope Nikon Tie, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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light microscope nikon tie - by Bioz Stars, 2026-03
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tie n-sim microscope  (Nikon)
90
Nikon tie n-sim microscope
The CSPG4 and perlecan interaction is involved in cell adhesion and spreading. (A) MM200 cell adhesion on fibronectin coated surface was used as a positive control (i). MM200 cell adhesion on HCAEC perlecan coated surfaces, in the absence of antibody (ii), in the presence of antibodies against α2β1 integrin clone BHA2.1 (iii), against perlecan clone A74 (iv) and against CSPG4 clones B5 (v) and clone 9.2.27 (vi). Cells were stained for actin filaments with Rhodamine-phalloidin (i–vi) and imaged using confocal <t>microscopy.</t> White arrows point out the polymerized actin filaments. Scale bar represents 10 µm. (B) Live cell images using <t>DIC</t> were also taken after MM200 cell adhesion on HCAEC perlecan coated surfaces in the absence of antibody (i), in the presence of antibody against CSPG4 clones B5 (ii) and clone 9.2.27 (iii) for 1 h. Scale bar represents 50 µm. (C) Analysis of the perimeter of cells exposed to the perlecan coating for 1 h in the absence or presence of antibodies from (B). A minimum of 10 cells were analysed from three confocal images taken at random for each condition. Individual data points are indicated as well as mean ± standard deviation. (D) The number of cells adhered to the perlecan coating after 1 h in the absence or presence of anti-CSPG4 antibodies determined from the live cell DIC images in (C). Individual data points were indicated as well as mean ± standard deviation. ‘*’ denoted significant differences compared to cells plated on perlecan in the absence of antibodies (P < 0.05) analysed by one-way ANOVA. Data shown are representative of three independent experiments.
Tie N Sim Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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laminin 332 β3 6f12 monoclonal antibody  (OriGene)
90
OriGene laminin 332 β3 6f12 monoclonal antibody
Regeneration of a Functional Transgenic Epidermis (A and B) In situ hybridization with a vector-specific probe on 20-μm-thick skin sections shows the homogeneous expression of laminin 332-β3 transcripts in all epidermal layers (B, arrowheads). Sections from normal skin were used as a control (A). Dotted lines indicate the basal lamina. Asterisks mark the stratum corneum. Scale bars, 10 μm. (C–F) Light microscopy of 0.5 μm sections from a skin biopsy of the upper leg of a healthy donor (C and E) and Claudio (D and F) were stained with toluidine blue. In both cases, normal-looking epidermis (Ep) and dermis with well-organized collagen bundles (c) are evident. Asterisks mark the stratum corneum, which is thicker in the regenerated epidermis. Scale bars, 10 μm. (G and H) Transmission electron microscopy of 70 nm skin sections shows that basement membranes (arrowheads) and hemidesmosomes (arrows) are clearly evident in both control (G) and transgenic (H) skin. Scale bars, 1 μm.
Laminin 332 β3 6f12 Monoclonal Antibody, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tie light microscopes  (Nikon)
99
Nikon tie light microscopes
Regeneration of a Functional Transgenic Epidermis (A and B) In situ hybridization with a vector-specific probe on 20-μm-thick skin sections shows the homogeneous expression of laminin 332-β3 transcripts in all epidermal layers (B, arrowheads). Sections from normal skin were used as a control (A). Dotted lines indicate the basal lamina. Asterisks mark the stratum corneum. Scale bars, 10 μm. (C–F) Light microscopy of 0.5 μm sections from a skin biopsy of the upper leg of a healthy donor (C and E) and Claudio (D and F) were stained with toluidine blue. In both cases, normal-looking epidermis (Ep) and dermis with well-organized collagen bundles (c) are evident. Asterisks mark the stratum corneum, which is thicker in the regenerated epidermis. Scale bars, 10 μm. (G and H) Transmission electron microscopy of 70 nm skin sections shows that basement membranes (arrowheads) and hemidesmosomes (arrows) are clearly evident in both control (G) and transgenic (H) skin. Scale bars, 1 μm.
Tie Light Microscopes, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
tie light microscopes - by Bioz Stars, 2026-03
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Image Search Results


The CSPG4 and perlecan interaction is involved in cell adhesion and spreading. (A) MM200 cell adhesion on fibronectin coated surface was used as a positive control (i). MM200 cell adhesion on HCAEC perlecan coated surfaces, in the absence of antibody (ii), in the presence of antibodies against α2β1 integrin clone BHA2.1 (iii), against perlecan clone A74 (iv) and against CSPG4 clones B5 (v) and clone 9.2.27 (vi). Cells were stained for actin filaments with Rhodamine-phalloidin (i–vi) and imaged using confocal microscopy. White arrows point out the polymerized actin filaments. Scale bar represents 10 µm. (B) Live cell images using DIC were also taken after MM200 cell adhesion on HCAEC perlecan coated surfaces in the absence of antibody (i), in the presence of antibody against CSPG4 clones B5 (ii) and clone 9.2.27 (iii) for 1 h. Scale bar represents 50 µm. (C) Analysis of the perimeter of cells exposed to the perlecan coating for 1 h in the absence or presence of antibodies from (B). A minimum of 10 cells were analysed from three confocal images taken at random for each condition. Individual data points are indicated as well as mean ± standard deviation. (D) The number of cells adhered to the perlecan coating after 1 h in the absence or presence of anti-CSPG4 antibodies determined from the live cell DIC images in (C). Individual data points were indicated as well as mean ± standard deviation. ‘*’ denoted significant differences compared to cells plated on perlecan in the absence of antibodies (P < 0.05) analysed by one-way ANOVA. Data shown are representative of three independent experiments.

Journal: Journal of Biochemistry

Article Title: Cell surface chondroitin sulphate proteoglycan 4 (CSPG4) binds to the basement membrane heparan sulphate proteoglycan, perlecan, and is involved in cell adhesion

doi: 10.1093/jb/mvy008

Figure Lengend Snippet: The CSPG4 and perlecan interaction is involved in cell adhesion and spreading. (A) MM200 cell adhesion on fibronectin coated surface was used as a positive control (i). MM200 cell adhesion on HCAEC perlecan coated surfaces, in the absence of antibody (ii), in the presence of antibodies against α2β1 integrin clone BHA2.1 (iii), against perlecan clone A74 (iv) and against CSPG4 clones B5 (v) and clone 9.2.27 (vi). Cells were stained for actin filaments with Rhodamine-phalloidin (i–vi) and imaged using confocal microscopy. White arrows point out the polymerized actin filaments. Scale bar represents 10 µm. (B) Live cell images using DIC were also taken after MM200 cell adhesion on HCAEC perlecan coated surfaces in the absence of antibody (i), in the presence of antibody against CSPG4 clones B5 (ii) and clone 9.2.27 (iii) for 1 h. Scale bar represents 50 µm. (C) Analysis of the perimeter of cells exposed to the perlecan coating for 1 h in the absence or presence of antibodies from (B). A minimum of 10 cells were analysed from three confocal images taken at random for each condition. Individual data points are indicated as well as mean ± standard deviation. (D) The number of cells adhered to the perlecan coating after 1 h in the absence or presence of anti-CSPG4 antibodies determined from the live cell DIC images in (C). Individual data points were indicated as well as mean ± standard deviation. ‘*’ denoted significant differences compared to cells plated on perlecan in the absence of antibodies (P < 0.05) analysed by one-way ANOVA. Data shown are representative of three independent experiments.

Article Snippet: Images were taken with differential interference contrast (DIC) microscopy (Nikon Eclipse TiE).

Techniques: Positive Control, Clone Assay, Staining, Confocal Microscopy, Standard Deviation

Regeneration of a Functional Transgenic Epidermis (A and B) In situ hybridization with a vector-specific probe on 20-μm-thick skin sections shows the homogeneous expression of laminin 332-β3 transcripts in all epidermal layers (B, arrowheads). Sections from normal skin were used as a control (A). Dotted lines indicate the basal lamina. Asterisks mark the stratum corneum. Scale bars, 10 μm. (C–F) Light microscopy of 0.5 μm sections from a skin biopsy of the upper leg of a healthy donor (C and E) and Claudio (D and F) were stained with toluidine blue. In both cases, normal-looking epidermis (Ep) and dermis with well-organized collagen bundles (c) are evident. Asterisks mark the stratum corneum, which is thicker in the regenerated epidermis. Scale bars, 10 μm. (G and H) Transmission electron microscopy of 70 nm skin sections shows that basement membranes (arrowheads) and hemidesmosomes (arrows) are clearly evident in both control (G) and transgenic (H) skin. Scale bars, 1 μm.

Journal: Stem Cell Reports

Article Title: Long-Term Stability and Safety of Transgenic Cultured Epidermal Stem Cells in Gene Therapy of Junctional Epidermolysis Bullosa

doi: 10.1016/j.stemcr.2013.11.001

Figure Lengend Snippet: Regeneration of a Functional Transgenic Epidermis (A and B) In situ hybridization with a vector-specific probe on 20-μm-thick skin sections shows the homogeneous expression of laminin 332-β3 transcripts in all epidermal layers (B, arrowheads). Sections from normal skin were used as a control (A). Dotted lines indicate the basal lamina. Asterisks mark the stratum corneum. Scale bars, 10 μm. (C–F) Light microscopy of 0.5 μm sections from a skin biopsy of the upper leg of a healthy donor (C and E) and Claudio (D and F) were stained with toluidine blue. In both cases, normal-looking epidermis (Ep) and dermis with well-organized collagen bundles (c) are evident. Asterisks mark the stratum corneum, which is thicker in the regenerated epidermis. Scale bars, 10 μm. (G and H) Transmission electron microscopy of 70 nm skin sections shows that basement membranes (arrowheads) and hemidesmosomes (arrows) are clearly evident in both control (G) and transgenic (H) skin. Scale bars, 1 μm.

Article Snippet: IF was performed on 7 μm skin sections as previously described ( ) using laminin 332-β3 6F12 monoclonal antibody (mAb; Acris Antibodies), 332-γ2 D4B5 mAb (Chemicon), 332-α3 BM165 mAb (a gift from Patricia Rousselle, IBCP), 332-α6 450-30A mAb and 332-β4 450-9D mAb (Thermo Fisher Scientific), rabbit purified anti-p63α immunoglobulin G (IgG; PRIMM) , K10 and K14 guinea pig antisera (Progen), K9 sc-58743 mAb (Santa Cruz Biotechnology), elastin MAB2503 mAb (Millipore), and human involucrin mAb (Leica Biosystems).

Techniques: Functional Assay, Transgenic Assay, In Situ Hybridization, Plasmid Preparation, Expressing, Light Microscopy, Staining, Transmission Assay, Electron Microscopy

Expression of LAM332 and α6β4 Integrins (A–J) IF analysis of laminin 332-β3 (A and B), 332-γ2 (C and D) 332-α3 (E and F), α6 integrin (G and H), and β4 integrin (I and J) in control (WT) and transgenic (Claudio) skin sections. The transgenic epidermis expresses normal amounts of laminin 332 and α6β4 integrins properly located at the epidermal-dermal junction. Scale bars, 40 μm.

Journal: Stem Cell Reports

Article Title: Long-Term Stability and Safety of Transgenic Cultured Epidermal Stem Cells in Gene Therapy of Junctional Epidermolysis Bullosa

doi: 10.1016/j.stemcr.2013.11.001

Figure Lengend Snippet: Expression of LAM332 and α6β4 Integrins (A–J) IF analysis of laminin 332-β3 (A and B), 332-γ2 (C and D) 332-α3 (E and F), α6 integrin (G and H), and β4 integrin (I and J) in control (WT) and transgenic (Claudio) skin sections. The transgenic epidermis expresses normal amounts of laminin 332 and α6β4 integrins properly located at the epidermal-dermal junction. Scale bars, 40 μm.

Article Snippet: IF was performed on 7 μm skin sections as previously described ( ) using laminin 332-β3 6F12 monoclonal antibody (mAb; Acris Antibodies), 332-γ2 D4B5 mAb (Chemicon), 332-α3 BM165 mAb (a gift from Patricia Rousselle, IBCP), 332-α6 450-30A mAb and 332-β4 450-9D mAb (Thermo Fisher Scientific), rabbit purified anti-p63α immunoglobulin G (IgG; PRIMM) , K10 and K14 guinea pig antisera (Progen), K9 sc-58743 mAb (Santa Cruz Biotechnology), elastin MAB2503 mAb (Millipore), and human involucrin mAb (Leica Biosystems).

Techniques: Expressing, Transgenic Assay